Figure 3 | Scientific Reports

Figure 3

From: Neutrophil extracellular traps promote erectile dysfunction in rats with diabetes mellitus by enhancing NLRP3-mediated pyroptosis

Figure 3

NLRP3 expression and pyroptosis were enhanced in the corpus cavernosum tissues of diabetic ED rats. (A) Assessment of NLRP3 expression in the corpus cavernosum tissues of rats using immunohistochemistry (n = 6/group; scale bar = 50 μm). (B) Assessment of the protein expression levels of NLRP3, GSDMD, GSDMD-N, pro-caspase-1, cleaved-caspase-1, and ASC in the corpus cavernosum tissues of rats using western blotting (n = 6/group); the uncropped protein blots can be seen in Supplementary file 1. (C) Assessment of plasma IL-1β and IL-18 levels in rats using ELISA (n = 6/group). Data were expressed as mean ± standard deviation. **p < 0.01. NLRP3 NOD-like receptor family, pyrin domain-containing protein 3, ED erectile dysfunction, GSDMD gasdermin D, GSDMD-N N-terminal proteolytic fragment of gasdermin D, ASC apoptosis-associated speck-like protein containing a CARD, IL interleukin, ELISA enzyme-linked immunosorbent assay.

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