Figure 5

In vivo histopathological observation. (A) H&E staining of PU wound site of differernt groups. (B) Masson's trichrome staining and magnification images. (C) Immunofluorescence analysis of CD31 of the wound site. (D) Immunohistochemistry staining images of Col-1 of the wound site. Scale bar = 200 μm. (E) Quantitative analysis of collagen ratio. (F) Average fluorescence intensity score of CD31. (G) Quantitative analysis of Col-1 expression. Data expressed as mean ± SD. *p < 0.05 or **p < 0.01 vs. control group by one-way ANOVA followed by LSD multiple comparison. We repeated the experiments three times to ensure the accuracy of the experiments.