Figure 1
From: Pituitary-derived small extracellular vesicles promote liver repair by its cargo miR-143-3p
Characterization of MMQ-sEV and its effects on BRL 3A cells proliferation. (a) Analysis of MMQ-sEV by transmission electron microscopy. (b) Nanoparticle tracking analysis of size distribution of isolated MMQ-sEV. (c) Western Blot analysis showing the biomarkers of sEV including CD9, CD63 and TSG101. Calnexin was used as negative control. (d–g) Effects of MMQ-sEV on BRL-3A cells proliferation. The BRL 3A cells proliferation was evaluated with (d) CCK8 kits, (e) EdU-positive cells, (f) PCNA mRNA and (g) PCNA protein expression.
