Figure 5

Specific splicing factors may regulate global “skipped exon” events. (A) Specific motif sequences used to search for binding sites in the vicinity of the skipped exon for the splicing regulators shown in panels (B) and (C) and key to the binding profiles of RBPs traced in the graphs. The solid lines are motif scores (Y axis, left side), calculated as the overall percentage of nucleotides covered by the motif in a 50 bp window, with red denoting the motif density score for exons with increased inclusion (38 events), blue for exons with increased skipping (22 events), and black for background (non-regulated) exons (70 events). The dotted lines are − log10 (P-value) (Y-axis, right side) based on comparison of motif scores between regulated exons against background exons. Only -log10 (P-value) greater than 1.3 are considered. The green exon is the target exon (that is skipped or included) and the flanking exons are shown in grey. (B) Maps of the RBPs SRSF1, Sam68 (KHDRBS1), and IGF2BP2, which are known to play a role in adipogenesis. (C) Maps of novel RBPs ESRP1, LIN28A, CPEB4, PABPC1, KHDRBS2, and HNRNPA1 that could alter the global alternative splicing profile during adipogenesis. (D) Summary of binding profiles of the RBPs, with proteins promoting inclusion indicated as red circles and those promoting skipping as blue circles.