Figure 2 | Scientific Reports

Figure 2

From: Screening and identification of miRNAs negatively regulating FAM83A/Wnt/β-catenin signaling pathway in non-small cell lung cancer

Figure 2

miR-1 directly targets FAM83A. (A) The expression of FAM83A by western blotting analysis after predicted miRNAs transfected in A549 for 48 h. GAPDH was used as a control. Blots were made from the same gel. (B) Quantification of FAM83A/GAPDH fold changes in Control and screened miRNAs. (C) Schematic illustration of predicted miR-1 binding sites within the 3’-UTR of FAM83A mRNA (position 212–219). (D) Luciferase activity was measured in lysates of HEK293T cells transfected with the wild-type 3′-UTR and mutant 3′-UTR of FAM83A luciferase constructs and a miR-1 mimic or a scrambled miRNA control. The luciferase activity was normalized to renilla luciferase activity. (E) miR-1 levels were inversely correlated with the levels of FAM83A in TCGA lung cancer database. (F) Relative mRNA levels of FAM83A after transfected with miR control and miR-1 mimics in A549 cells. All data are representative of 3 independent experiments. Data are represented as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001.

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