Figure 3

miR-1 decreases Wnt/β-catenin signal through FAM83A. (A) Western blotting analysis of Wnt/β-catenin signal target genes C-MYC, CyclinD1 and AXIN2 in A549 and H1299 cell lysates with or without miR-1 overexpression and re-expression FAM83A or vector. Representative western blotting images of 3 independent experiments. GAPDH was used as a control. Blots were made from the same gel. (B–D) The schematic diagram of 7TGC plasmid construction and the fluorescence images of HEK-293 T cells with or without miR-1 overexpression and re-expression FAM83A or vector. The relative ratio of green/red fluorescence intensity was quantified. (E–J) The mRNA level of Wnt/β-catenin signal target genes C-MYC, CyclinD1 and AXIN2 in A549 and H1299 cells with or without miR-1 overexpression and re-expression FAM83A or vector. (K) The protein level of cytoplasmic and nucleus β-catenin in HEK-293 T cells with or without miR-1 overexpression and re-expression FAM83A or vector were analyzed by western blotting. GAPDH was used as a cytoplasmic control. Histone H3 (H3) was used as a nucleus control. Representative western blotting images of 3 independent experiments. Blots were made from the same gel. (L, M) Distribution of β-catenin in A549 cells with or without miR-1 overexpression and re-expression FAM83A or vector. The relative ratio of nucleus/cytoplasm fluorescence intensity was quantified. Data are representative of 3 independent experiments. Data are represented as mean ± SD (n = 3). *p < 0.05, **p < 0.01,***p < 0.001.