Figure 3

(A–C) Immunohistochemical analysis of cleaved caspase-3 in animals treated with ECT and i.v. BLM (A), i.t. BLM (B), or i.v. + i.t. (C) administration of BLM. Apoptotic cells (arrows) are stained red. Scale bars: 50 µm. (D) The diagram displays the mean number of positive cells in the tumor tissue per HPF. Data are given as mean ± SEM.