Figure 1
From: Identification of kinase inhibitors as potential host-directed therapies for intracellular bacteria
Identification of PKIS compounds inhibiting intracellular growth of Stm and Mtb. (a) Gating strategy for DsRed-bright Stm-infected HeLa cells to determine the percentage of infected cells. The negative control treated with DMSO and positive control treated with 97i are depicted in grey. (b) Rescreen of 201 PKIS compounds, 30 of which appear twice, to assess their impact on Stm bacterial burden, expressed as average z-scores of the DsRed-bright population. (c) Hit compounds with z-scores < − 2. PKIS compounds that reduced bacterial burden in both the primary screen and rescreen are depicted in green, while black dots represent compounds that were only effective in one of the two screens. Controls are depicted in grey. (d) Gating strategy for DsRed+ Mtb-infected MelJuSo cells. (e) Re-screen on Mtb-infected MelJuSo cells. (f) Hit compounds with z-scores < − 2 for Mtb. The screens were performed with three technical replicates, and error bars show standard deviations.
