Figure 3

Distribution plot of read counts for the 56 African buffalo tissue samples and each genetic target (hsp65, rpoB, and 16S rRNA). Reference-free sorting and assembly of consensus sequences were performed on a maximum of 200,000 randomly selected reads (> Q12) for each sample. For tissue samples with fewer reads, the entire available dataset was included. Reads were generated using Oxford Nanopore Technologies (ONT) on selected targeted genes shown on the x-axis. Singletons that could not be included in any group because of their length or sequence similarity are reported as unassigned and were excluded from the analysis.