Figure 3
Semi-quantitative phage spot assays for determination of phage susceptibility. S. marcescens strains were grown in LB to an OD600 of 1.0, mixed with molten LB with 0.5% agar, and poured on an LB 1.5% agar plate. Phage χ dilutions in 0.85% NaCl were spotted on the solidified agar surface, and plates were incubated at 25 °C overnight and imaged using a Cytiva IQ800 instrument. Zones of clearing indicate lysis and thus χ susceptibility. Dilutions are as follows: top row left to right: 100, 10−1, 10−2; bottom row left to right: 10−4, 10−5, 10−6.
