Figure 5
(A) Plates of S. marcescens ΔfliC liquid cultures with added χ phage lysate, Δpig cell supernatant, or fresh LB medium at a ratio of 1:1. After 18 h of incubation at 25 °C with shaking, 5 ml of each culture was pipetted into one well of a 6-well rectangular plate. Plates were imaged with a Cytiva IQ800 instrument. (B) Pigmentation in S. marcescens ΔfliC cultures as A535 quantified via spectrophotometer 18 h after addition of a χ phage lysate, Δpig cell supernatant, Δpig culture mechanical lysate, or fresh LB medium to cultures in exponential phase (OD600 = 0.5) or stationary phase (OD600 = 2.0). Data points are mean values consisting of three replicates. Error bars indicate standard deviation. Student’s t-test was used to determine statistical significance. P values are represented by asterisks: *P < 0.05; **P < 0.01; ***P < 0.001.
