Table 1 Strains, plasmids, and phage used in this study.
Strain, plasmid, or phage | Parental strain/backbone | Relevant characteristics | Source |
|---|---|---|---|
Serratia marcescens ATCC 274 (WT) | ATCC 274 | Wild type | Rasika Harshey |
ΔfliC | ATCC 274 | ΔfliC (flaF) | This study |
ΔluxS | ATCC 274 | ΔluxS | This study |
Δpig | ATCC 274 | ΔpigABCDEFGHIJKLMN | This study |
ΔpigP | ATCC 274 | ΔSMATCC274_40570 | This study |
pig-lacZ | ATCC 274 | ΔfliC; pigA::lacZ | This study |
pigq | ATCC 274 | Ppig replaced with J23119 | This study |
pKNG101 | – | MCS, strAB, sacB, mobRK2, oriV R6Kγ, traJK | Sarah J Coulthurst |
pBS1315 | pUC18 | Cloning vector with J23119 promoter | This study |
pBS1355 | pKNG101 | fliC (flaF) deletion construct | This study |
pBS1359 | pKNG101 | pigq mutant construct | This study |
pBS1361 | pKNG101 | pig operon deletion construct | This study |
pBS1362 | pKNG101 | pig-lacZ fusion mutant construct | This study |
DH5α-λpir | Escherichia coli K-12 | F– φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK–, mK+) phoA supE44 λ–thi-1 gyrA96 relA1 LAMpir | Howard C. Berg |
SM10-λpir | Escherichia coli K-12 | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu KmLAMpir | Clay Fuqua |
χ (Chi) | – | Wild type | Saeed Tavazoie |
