Figure 2
From: The involvement of cyclotides in the heavy metal tolerance of Viola spp.
TEM microphotographs of V. tricolor cells from suspensions treated with 2000 µM of Pb (A–D) or Zn (E–H) for 72 h and control cells (I–K). The distribution maps show cell walls (CW) and vacuoles (V) treated with Pb (A) and Zn (E) that co-localize with lead (B, green points) or Zn (F, light blue points) deposits, gold particles (representing cyclotides) (C, G and J, red points) and sulfur deposits (D, H and K, yellow points). The circles show examples of co-localization sites for metals, sulfur and cyclotides. All treatments were prepared in two biological and three analytical replicates.
