Table 2 The basic characteristics of cyclotides found in V. lutea ssp. westfalica callus tissue transcriptome—amino acid sequence and calculated molecular weights (MW).

Viul D

GIPCGESCVWIPCLTSAIGCSCKSKVCYKN

3124.32

viul M

GIPCGESCVFIPCLTAAIGCSCKSKVCYRN

3097.32

vitri A

GIPCGESCVWIPCITSAIGCSCKSKVCYRN

3152.33

vitri 98

GIPCGETCVFSGCYSVTFGCACEKRVCYKNu

3199.26

Part of vitri 60 precursor

GIPCGETCIFGRCHTGIIGCACEKYMCCKN

3185.24

vitri 42a

GGTIFNCGESCFQGTCYTKGCACGDWKLCYGEN

3433.25

vitri 28 (incomplete)*

VPSSDCLETCFGGKCNAHRCTCSQWPLCAKN

3333.32

vinc A (incomplete)*

PVCGETCTLGTCYTAGCSCSWPVCTRN

2787.01

varv peptide F

GVPICGETCTLGTCYTAGCSCSWPVCTRN

2957.12

varv peptide D

GLPICGETCVGGSCNTPGCSCSWPVCTRN

2876.07

Kalata S

GLPVCGETCVGGTCNTPGCSCSWPVCTRN

2876.07

Kalata B1 (incomplete)*

LPVCGETCVGGTCNTPGCTCSWPVCTRN

2833.07

CyO8

GTLPCGESCVWIPCISSVVGCSCKSKVCYKN

3225.37

CyO4

GIPCGESCVWIPCISSAIGCSCKNKVCYRN

3165.33

CyO3

GIPCGESCVWIPCLTSAIGCSCKSKVCYRN

3152.33

CyO12

GLPICGETCVGGTCNTPGCSCSWPVCTRN

2890.09

viwe 1

GLPVCGETCVGGPCNTPGCSCSRPVCTRN

2842.10

viwe 2

GLPICGETCVGGTCNTPGCICSWPVCTTN

2861.09

viwe 3

GIPICGETCVGGTCNTPGCSCSWPVCVRN

2888.11

viwe 4

GIPICGETCVGGTCNTPGCSCSWPVCTRN

2890.09

viwe 5

GLPVCGETCVGGTCNTLGCSCSWPVCTRN

2892.11

viwe 6

GMPVCGETCVGGTCNTPGCSCSWPVCTRN

2894.03

viwe 7

GLPVCGETCVGGTCNTPGCSCSWPVCKRN

2903.12

viwe 8

GLPICGETCVGGSCNTPGCSCSWPMCVRN

2906.07

viwe 9

GLPVCGETCVGGTCNTPGCSCSWPVCMRN

2906.07

viwe 10

GLPICGETCVGGTCNTPGCSCSWPVCMRN

2920.08

viwe 11

GIPICGETCTLGTCYTAGCSCSWPVCTRN

2971.14

viwe 12

GLPICGETCTLGTCYTAGCSCSWPVCTRN

2971.14

viwe 13

GVPICGETCTLGTCYTAGCTCSWPVCTRN

2971.14

viwe 14

GHCGESCMVLPCFTASRGCSCSGAICWKN

2982.11

viwe 15

GVPICGDTCFGGTCYTPGCSCSWPVCMRN

2989.07

viwe 16

SIPCGESCVFIPCLTGAIGCACKSSVCYLN

3013.24

viwe 17

GSAVPCGESCFFGGGCDTPGCSCTWPACTKN

3017.05

viwe 18

SIPCAESCAFVPCYGIIPCSCKNGICYSN

3017.18

viwe 19

GLPVCGETCLGGTCNTPGCSCSWPVCVKYD

3024.15

viwe 20

GSIFNCGETCILGTCYTPGCSCVYGACSKN

3026.13

viwe 21

GSIFNCGESCVLGTCYTSGCSCVYGLCSKN

3030.13

viwe 22

GSIFNCGESCVLGTCYTPGCSCVYGLCSKN

3040.15

viwe 23

GVACPETCIFTSCFITSCTCDHGRCRRN

3055.19

viwe 24

GIPCGESCVFIPCLTAAIGCSCSSKVCYRN

3056.26

viwe 25

GYPCVETCVFSGCFITNCICNYGSCVWN

3057.12

viwe 26

GSVFNCGESCLGGKCNTPDCTCSFPLCTKN

3060.15

viwe 27

GSVFNCGESCLGGTCNTPGCTCSSFPLCTKN

3062.13

viwe 28

GIPCAETCAFIPCMATAVFGCSCSNNVCYN

3064.13

viwe 29

GSIPCAESCAFVPCYGIIPCSCKNGICYSN

3074.20

viwe 30

GWSCQETCIFSSCYLTGCTCSYSVCKKN

3076.15

viwe 31

GIPCGESCVWIPCITAAIGCSCSSNVCYRN

3081.22

viwe 32

GIPCGESCVFIPCITAAIGCSCSNKVCYRN

3083.27

viwe 33

GIPCGESCVFIPCLTAAIGCSCSNKVCYRN

3083.27

viwe 34

GSVFNCGETCIWGTCYTPGCSCVYGACSKN

3085.11

viwe 35

GIPCGESCVCITCISSAIGCSCKIKVCYRN

3085.28

viwe 36

GIPCAESCVWIPCTITALLGCGCSNKVCYN

3093.28

viwe 37

GVPTCDESCVLGTCFTPDCTCSWPICLRN

3095.19

viwe 38

GIPCGESCVWIPCISSVIGCSCSSKVCYKN

3097.28

viwe 39

GQFCGESCIVSSCYITRCTCTANFCYRN

3104.16

viwe 40

GIPCGESCVWIPCISSVVGCSCSSKVCYRN

3111.27

viwe 41

GLYVCGERCLRGRCNAPGCICTNKICTKN

3120.36

viwe 42

GRFCLETCVFSSCFITGCDCEFTSCFKN

3122.17

viwe 43

GIPCGESCVWIPCITAAIGCSCSNKVCYRN

3122.28

viwe 44

GIPCGESCVWIPCVTVVIGCSCSSNVCYRN

3123.27

viwe 45

GIPCAESCVWIPCTITALLGCSCSNKVCYN

3123.29

viwe 46

GSIFKCGETCVLGTCYTPGCHCIWGVCAKN

3125.26

viwe 47

GVLPCGESCVFIPCVTAVIGCACKSSVCYKN

3125.34

viwe 48

GSSCYESCYLIPCITSIAGCSCNQNTCTDD

3126.09

viwe 49

GDVCTETCFTDYCFLGGCTCYWPVCKKN

3131.16

viwe 50

GPLCGDTCVYDPCLISAPCKCKNKVCYRN

3138.31

viwe 51

GSIFNCGETCLLGTCYTSGCSCVYRVCSKD

3144.20

viwe 52

GIPCGETCLFGGCNTSIFGCACEKRVCYKN

3148.26

viwe 53

GSIPCGESCVWIPCISGIAGCSCSNKVCYKN

3153.28

viwe 54

GIPCGITCELNPCHSFVPCTCQHRVCYSN

3156.24

viwe 55

GVPCGESCVFNPCLTGVVLCRCSSYVCFKD

3160.29

viwe 56

GSVPCGESCVWIPCISAVVGCSCSNKVCYKN

3167.29

viwe 57

GSTPCGESCVWIPCISAVVGCSCSNKVCYKN

3169.27

viwe 58

GSTPCGESCVWIPCISSVVGCSCSNKVCYLN

3170.26

viwe 59

GSTPCGESCVWIPCISAVVGCSCSNKVCYMN

3172.22

viwe 60

GRVPCGETCSLGTCYFAGCTCDWPICWRN

3173.20

viwe 61

GSTPCGESCVWIPCISSIVGCSCSSKVCYMN

3175.22

viwe 62

GLTCVESCIVIPCVTGLLGCYCSNHICYKN

3180.35

viwe 63

GSIPCGESCVWIPCISAVVGCSCSNKVCYKN

3181.31

viwe 64

GIPCGESCVWIPCLTSTIGCSCKSKVCYRN

3182.34

viwe 65

GVGAYCGESCVLIPCLSAIIGCSCSNSDCFKN

3188.27

viwe 66

SIPCAESCVWIPCISSVVGCSCKSKVCYRN

3196.36

viwe 67

GIPCGETCIFSGCYSVTFGCACEKRVCYKN

3213.28

viwe 68

GVLPCGESCVWIPCISSVVGCSCKSKVCYKN

3223.39

viwe 69

GFSCMESCILLPCATKYIGCSCENKLCVKN

3232.35

viwe 70

GSSKCWETCVLIPCATAILGCSCKDFICVKN

3267.42

viwe 71

GIPCGESCVWIQCISSAIGCYCNNKVCFWN

3272.29

viwe 72

GLVSCGQSCYRTPCVALTTCSCRFHVCYKN

3274.35

viwe 73

GTIFDCGESCLLGKCYTPGCACGSWGLCYGQN

3281.23

viwe 74

GTIFDCGESCVFGTCYTPGCACGSWALCYGQN

3288.17

viwe 75

GTFPCGESCVWIPCLSKVIGCACKSKVCYKN

3298.44

viwe 76

GLNCGETCWGFSCDRDDCSCWTWPYCSKN

3311.12

viwe 77

GTIFDCGESCLLGTCYTKGCSCGSWKLCYGTN

3345.28

viwe 78

GWLHCGETCRITPCLAADLMCFCTDGVCLRN

3376.37

viwe 79

GSQHCAETCYLIPCLTTQIGCSCINRACYRN

3396.39

viwe 80

SLFNCGETCLWGTCYTPGCNCNKWRVCEKN

3404.32

viwe 81

GWISCVEACYYLPCASRVFGCSCVRNVCMRN

3464.41

viwe 82

GTIFNCGESCFHGKCYTKGCACGDWKLCYGEN

3469.30

viwe 83

GEHCYEVCYFNPCVTRLLGCYCHLHMCIKN

3523.41

viwe 84

GQARFCHETCTLNPRCITAQFGCYCTHRVCTIN

3721.55