Figure 5
Neuro score, liquid intake, and carprofen plasma concentration. Relevant Neuro score parameters at the 4 h time point and on day 1 are shown as percentage mean increase (highlighted in red) or decrease (highlighted in blue) for individual scores per sex, time point, and analgesic regimen in (a). While the liquid intake (b) significantly increased in most female mice on day -1, it showed a decline on day 0 for female surgery + NO and + NLO mice. Data shown for BL_mean represents the individual, mean water intake over five days during baseline measurements (group sizes differing from n = 8: males day 4 surgery + N n = 7; females day 0 drug-control + NL n = 7). The carprofen plasma concentrations measured on day 4 (c) exceeded the hypothesized therapeutic plasma level of 20–24 µg/ml in both male and female mice (group sizes differing from n = 8: males naive-control n = 7, surgery + N n = 7, drug-control + NL n = 7, surgery + NO n = 6; females: naive-control n = 7, drug-control + NL n = 7, drug-control + NO n = 7). (* = p < 0.05, analgesic regimen color-coded in (b); mean ± SD in (b) and (c); Two-way RM ANOVA with Bonferroni post hoc in (b)).
