Figure 7

Activity of Trypanothione Synthetase (TryS). Parasites (3 × 10⁷) were incubated with different concentrations of DhL, in the absence or in the presence of 3 mM GSH for 3 h and processed for TryS activity measurement as described in material and methods. Controls were performed with DMSO (< 0.05%) or with GSH alone. We used different concentration of H₂O₂ (0.5, 1 and 2 mM) as a positive control. Bars represent the specific enzymatic activity of TryS mU/mg protein from three independent experiments. (*)(***) indicate significant differences from each other (p < 0.05 and p < 0.01 respectively, obtained by Turkey–Kramer multiple comparisons test).