Figure 8
From: New insights into the pro-oxidant mechanism of dehydroleucodine on Trypanosoma cruzi
The effect of DhL on T. cruzi epimastigotes that overexpress reducing enzymes. Dm28c epimastigotes (1.5 × 106 cells/mL) that overexpress; mTXNPx, cTXNPx, Tc-GRx, and Tc-TXN II were incubated with different concentrations of DhL for 72 h. Then, the viability of the parasites was determined by resarzurin assay as described in material and methods. The curves represent the percentages of viability with respect to control (without drug, 100% of viability) at each DhL concentration. Each point on the curves represents the means ± SEM from three independent experiments. Parasites transfected with empty vector (pTEX) were used as controls. (*)(***)(****) indicate significant differences from the control (p < 0.05, p < 0.01and p < 0.001 respectively).
