Figure 2 | Scientific Reports

Figure 2

From: CHD4 R975H mutant activates tumorigenic pathways and promotes stemness and M2-like macrophage polarization in endometrial cancer

Figure 2

The CHD4 R975H mutation promotes a cancer stem cell (CSC)-like phenotype in endometrial cancer cells. (A) CHD4 expression levels in the constructed CHD4 WT (left) and shCHD4 (right) cell lines determined via qRT‒PCR. (B–F) Transwell, colony formation and MTT assays of the CHD4 R975H, CHD4 WT and shCHD4 cell lines. (B) Stained invasive cells in the transwell assay. (C) Quantification of invasive cells. (D) Stained colonies in the colony formation assay. (E) Quantification of colonies. (F) Absorbance of the chromogenic product formazan produced by live cells at 490 nm. The number of live cells is linearly related to the absorbance. (G) Tumor weight and volume in the mouse experiment.

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