Figure 4 | Scientific Reports

Figure 4

From: CHD4 R975H mutant activates tumorigenic pathways and promotes stemness and M2-like macrophage polarization in endometrial cancer

Figure 4

The CHD4 R975H mutant activates carcinogenic pathways and promotes the stemness of endometrial cancer cells. (A) Expression levels of marker genes in cancer-related pathways in the CHD4 R975H, CHD4 WT and shCHD4 cell lines measured by qRT‒PCR. (B) Expression levels of hallmark proteins involved in stemness (CD133), mTOR signaling (total S6K and pS6K) and TGF-βsignaling (β-catenin) in CHD4 R975H cells detected via western blotting. Original blots are presented in Supplementary Fig. 2. (C) Expression levels of stemness marker genes in the CHD4 R975H, CHD4 WT and shCHD4 cell lines measured by qRT‒PCR. (D) Flow cytometry analysis of CD133 staining in CHD4 R975H cell line. (E) Spheroids of Ishikawa and AN3-CA CHD4 R975H cell lines. (F) Number and diameter of spheroids.

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