Figure 5 | Scientific Reports

Figure 5

From: CHD4 R975H mutant activates tumorigenic pathways and promotes stemness and M2-like macrophage polarization in endometrial cancer

Figure 5

The CHD4 R975H mutation drives M2-like polarization in tumor-associated macrophages. (A) Abundance of M2 macrophages in tumor tissue of EC patients with or without CHD4 mutation. (B) Differential expression analysis of CCL and CXCL gene sets in tumor tissue of patients above. (C) Expression levels of M2 macrophage marker genes in the THP-1 cell line cocultured with the CHD4 R975H, CHD4 WT and shCHD4 cell lines measured by qRT‒PCR. (D) Flow cytometry analysis of CD68 + CD163/CD206 staining in cocultured THP-1 cell line. (E) Immunohistochemistry analysis of CD163/CD206 in tumor tissue of nude mice used in the mouse xenograft model. (F) Quantification of positive area of immunohistochemistry assay.

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