Figure 2
From: Dynamic cell culture modulates colon cancer cell migration in a novel 3D cell culture system
Cultivation chamber description and simulation of shear stress. (a) Schematic representation of the cultivation chamber and its layers: The cultivation chamber is made of different layers, a well for loading collagen gel and spheroids, a channel that allows medium to flow over the well. (b) Picture of the developed microscope stage holder (silver) with the cultivation chamber and connected tubing. Scale bar: 20 mm. (c) Online images showing HCT-116-GFP spheroids embedded in collagen I and cultured within the chamber under dynamic conditions for 6 days. Scale bar: 200 µm (d) Simulation of shear stress in the channel and over the well by COMSOL at flow rate of 1 ml/min.
