Figure 3
From: Dynamic cell culture modulates colon cancer cell migration in a novel 3D cell culture system
Investigation of migration for HCT-116-GFP in collagen I matrix in the dynamic 3D cell culture system. (a) Experimental workflow of spheroid generation by liquid overlay technique, embedding in collagen I matrix and analysis through microscopy and gene expression. (b) Representative images of spheroids cultured in liquid overlay (non-embedded (NES)), static or dynamic conditions illustrating the distinct core (dotted outline) and migration areas used for analysis. (c) Representative images of embedded spheroids in static and dynamic culture conditions. Quantification of spheroid migration on day 3 and day 7 (d), n = 8. Scale bar: 200 µm. Data are shown as mean ± SD (*p < 0.05).
