Figure 3 | Scientific Reports

Figure 3

From: Chemotherapy increases CDA expression and sensitizes malignant pleural mesothelioma cells to capecitabine treatment

Figure 3

Hypoxia increases CDA expression in MESO-1 cells over time and differentially affects the growth of MPM cell lines. (A) Western blot analysis of CDA and beta-actin protein expression in MESO-1 cells exposed to hypoxia for the indicated time points (B) Quantification of the data in Fig. 3A, i.e., CDA protein expression, normalized to the expression of beta-actin (ACTIN) relative to the untreated control on day 0. (C–F) Relative cell growth of the indicated cell lines. After the cells were seeded into 96-well plates, the plates were incubated in parallel under normoxic and hypoxic conditions for 72 h, after which the medium was replaced after 48 h. Cell growth was determined in parallel for all plates by the Presto-Blue assay. The data are presented as the mean ± SEM (n = 12). Not significant (ns), *p < 0.05 and ****p < 0.0001 according to an unpaired t test.

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