Figure 7 | Scientific Reports

Figure 7

From: Comprehensive analysis identifies ubiquitin ligase FBXO42 as a tumor-promoting factor in neuroblastoma

Figure 7

FBXO42 promotes neuroblastoma cell proliferation via TP53 dependence. (A) UbiBrowser 2.0 screening of potential substrate proteins for FBXO42. (B,C) GO (B) and KEGG (C) pathway enrichment analysis of 14 potential substrate proteins. (D) Immunoblotting of SK-N-SH or SH-SY5Y cell lines transfected with Flag-Control or Flag-FBXO42, using the indicated primary antibodies. (E,F) Colony formation assay (E) and CCK-8 (F) detecting the impact of Flag-FBXO42 on SK-N-SH proliferation. (G) SK-N-SH cell lines transfected with si-NC or si-FBXO42 were immunoblotted using the indicated primary antibodies. (H,I) Real-time quantitative PCR validation of si-TP53 (H) and si-FBXO42 (I) knockdown efficiency in SK-N-SH. (J,K) CCK-8 (J) and colony formation assay (K) assessing the effect of si-FBXO42 on SK-N-SH proliferation with or without TP53 inhibition.

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