Figure 2 | Scientific Reports

Figure 2

From: Hepatic stellate cells in zone 1 engage in capillarization rather than myofibroblast formation in murine liver fibrosis

Figure 2

SMMHC-CreERT2-labeled HSC are located in zone 1 of the hepatic lobule. (a) SMMHC-tdTomato reporter mice were injected with tamoxifen (tam) on 5 consecutive days to induce the expression of the fluorescent dye tdTomato under control of the SMMHC promoter (SMMHC-tdTomato). 30 days later, mice were sacrificed, and livers were then isolated, fixed and analyzed. tdTomato fluorescence is shown in red. (b) Antibodies against glutamine synthetase (GS, green) were used to identify hepatocytes around the central vein. (c) Antibodies against nerve growth factor receptor (NGFR, green) were used to identify portal vein-associated HSC according to Dobie et al.9. The enlargement in c4 shows tdTomato-negative, NGFR-positive cells towards the center of the lobule. Asterisks indicate central veins. Single channels are shown in b2/b3, c2/c3 and c5/c6.

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