Figure 4

TSC1 is a target gene of miR-27a-3p. (A) The Targetscan, starbase, and miRDB databases were used to predict the suspicious target for miR-27a-3p; (B) RT-qPCR analysis of relative expression of miR-27a-3p; (C) RT-qPCR was performed to detect the mRNA levels of fifteen possible target genes of miR-27a-3p; (D) The binding site between miR-27a-3p and the 3’UTR of TSC1 was predicted, and the mutation site (red) was constructed; (E) Transfection with antimiR-27a-3p apparently increased the relative luciferase activity in HEK-293 T cells; (F) RIP was performed to analyze the interaction between TSC1 and miR-27a-3p in HEK-293 T cells. (^***p < 0.001). TSC1 tuberous sclerosis complex 1; miR microRNA; RT-qPCR reverse transcription-quantitative polymerase chain reaction; HEK human embryonic kidney; RIP RNA immunoprecipitation.