Figure 1

Yeast two-hybrid (Y2H) analysis of protein interactions of VQ1 and VQ10 proteins with other hypoxia-, NO-, and oxidative stress-inducible VQ and WRKY proteins. Interactions were evaluated using a Y2H mating system, with yeast cells transformed with the various proteins either fused to the GAL4 binding domain (BD) or activation domain (AD). Control experiments to verify no interaction were conducted using empty (Ø) BD or AD clones.