Table 1 List of gRNAs designed with CRISPOR used in this article. Predicted specificity scores, efficiencies, outcomes and off-targets are indicated.
Target | Guide sequence + PAM | MIT specificity score | CFD Spec. score | Predicted efficiency score | Off-targets for 0–1–2–3–4 mismatches | |
|---|---|---|---|---|---|---|
Doench’16 | Mor-Mateos | |||||
Dup2 + Dup2–9 (sgRNA1) | 5′-GCATCTACATACTCTAACAT-3′ In intron1 | 81 | 93 | 61 | 35 | 0–0–0–5–84 |
Dup2 + Dup2–9 (sgRNA 2) | 5′-GTCAAACATGCATACTATGT-3′ In intron2 | 81 | 89 | 41 | 36 | 0–0–3– 8–96 |
Dup2 + Dup2–9 (sgRNA3) | 5′-GTCAAGATCTCAGTAACACT-3′ In intron2 | 80 | 91 | 63 | 35 | 0–0–3– 8–96 |
Dup8-9 (sgRNA A) | 5′-GTCAGATCAACTTAATGTGC-3′ In intron7 | 87 | 92 | 52 | 30 | 0–0–1–15–96 |
Dup8-9 (sgRNA B) | 5′-AGCTGCAAACTTTGTGTTCG-3′ In intron9 | 84 | 91 | 32 | 18 | 0–0–0–6–58 |
