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Figure 1

From: Fibroblast growth factor 2 promotes osteo/odontogenic differentiation in stem cells from the apical papilla by inhibiting PI3K/AKT pathway

Figure 1

Immunophenotypic characterisation and multiple differentiation analysis of SCAP. (A) Apical papilla from an extracted human third molar with developing root tissues. (B) Single fibroblastic cell attached one day after cell seeding. (C) SCAP grown in colonies. (D) Immunocytofluorescence staining of CD24 (green). (E) Immunocytofluorescence staining of vimentin (red). (F) Immunocytofluorescence staining of STRO-1 (green). (G) Keratin immunofluorescence staining (nuclear DAPI staining was blue). (H) ARS staining for mineralised nodules after SCAP cultured in an osteogenic medium for 3 weeks. (I) Positive staining for lipid droplets with ORO after adipogenic induction for 3 weeks. ARS Alizarin red S staining, SCAP stem cells from the apical papilla, ORO Oil Red O.

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