Figure 2 | Scientific Reports

Figure 2

From: Fibroblast growth factor 2 promotes osteo/odontogenic differentiation in stem cells from the apical papilla by inhibiting PI3K/AKT pathway

Figure 2

Impact of FGF2 pretreatment on osteo/odontogenesis differentiation of SCAP. SCAP were pretreated with FGF2 for 1 week in a standard medium. (A,B) FGF2 pretreatment enhanced mRNA and protein expression of DSPP, DMP1, and BGLAP. (C–E) Representative quantitative analysis of DSPP, DMP1, BGLAP protein (***P < 0.001). (F) FGF2 pretreatment enhanced ARS mineralisation. Scale bar = 200 μm. (G) Quantitative measurement of ARS staining (***P < 0.001). (H) FGF2 pretreatment enhanced ALP activity. Scale bar = 200 μm. (I) Quantitative analysis of ALP activity (***P < 0.001). ALP alkaline phosphatase staining, ARS Alizarin red S staining, FGF2 fibroblast growth factor 2, SCAP stem cells from the apical papilla.

Back to article page