Figure 3 | Scientific Reports

Figure 3

From: Fibroblast growth factor 2 promotes osteo/odontogenic differentiation in stem cells from the apical papilla by inhibiting PI3K/AKT pathway

Figure 3

PI3K/AKT pathway is involved in FGF2 stimulation of SCAP osteo/odontogenesis differentiation. SCAP were pretreated with either FGF2 and/or SC79 in the standard medium. (A) Clustered heatmap of RNA-seq, with columns representing different transcripts, and rows representing three biological replicates of FGF2-pretreated and untreated SCAP samples. (B) Volcano plot results of DEGs by each exposure groups. (C) RT-qPCR verified the differentially expressed PI3K/AKT pathway genes in the RNA-seq results (**P < 0.01, ***P < 0.001). (D) Gene Ontology enrichment analysis of differentially expressed mRNAs between FGF2 pretreated and untreated SCAP. (E) KEGG analysis of the signalling pathways involved in FGF2 pretreated and activated SCAP. FGF2 fibroblast growth factor 2, KEGG Kyoto Encyclopedia of Genes and Genomes, PI3K phosphoinositide three kinase, RNA-seq RNA sequencing, RT-qPCR quantitative reverse transcription polymerase chain reaction, SCAP stem cells from the apical papilla, DEG differentially expression gene.

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