Figure 4

FGF2 pretreatment promoted SCAP osteo/odontogenesis differentiation by the suppression of the PI3K/AKT signalling pathway. SCAP were pretreated with either FGF2 and/or SC79 in the standard medium. (A) FGF2 enhanced FGFR1 and FGFR2 expression (*P < 0.05). (B) AKT and p-AKT protein levels in SCAP pretreated with either FGF2 and/or SCA79. (C) Quantitative analysis of P-AKT/AKT in each groups (*P < 0.05, ***P < 0.001). (D) DSPP, DMP1, and BGLAP mRNA levels in SCAP were measured after subculture in induction medium for 1 week (*P < 0.05, ***P < 0.001). (E) DSPP, DMP1, and BGLAP protein levels in SCAP were measured after subculture in induction medium for 2 weeks. (F) Representative quantitative analysis of DSPP, DMP1, and BGLAP in each group (*P < 0.05, ***P < 0.001). (G) ARS staining in SCAP was measured after subculture in induction medium for 3 weeks. Scale bar = 200 μm. (H) Quantitative analysis of ARS staining (*P < 0.05). (I) ALP staining of SCAP was measured after subculture in induction medium for 1 week. Scale bar = 200 μm. (J) Quantitative analysis of ALP activity (*P < 0.05). ALP alkaline phosphatase staining, ARS Alizarin red S staining, FGF2 fibroblast growth factor 2, FGFR FGF receptor, PI3K phosphoinositide three kinase, RNA-seq RNA sequencing, SCAP stem cells from the apical papilla.