Figure 2

UBR1 interacts with YAP. (A) Immunofluorescence demonstrated the colocalization of UBR1 and YAP in CAL62 cells. (B) Co-IP indicated the interaction between UBR1 proteins with Flag tag (Flag-UBR1) and YAP proteins with Myc tag (Myc-YAP) in HEK 293T cells. (C) Immunoblotting demonstrated expression of YAP and UBR1 in both the cytoplasm and nucleus of CAL62 cells. (D) Co-IP indicated the interaction between UBR1 and YAP in cytoplasm.