Figure 3
Ergosterol inhibited the phosphorylation of AKT/GSK-3 β/β-catenin. (a) The effect of ER 20 μM on AKT phosphorylation in a time-dependent manner after 3, 6, 12, and 24 h. The western blot analysis of phospho-Akt (S473), phospho-β-catenin (T41/S45), (S33/S37/T41), (S552) and phospho-GSK-3α,β (S21/S9) was performed and analyzed after treated with ER (2 and 20 μM) for 6 h. Total Akt and β-actin were used to normalize the protein expression on (b) MCF-7 and (c) MDA-MB-231, respectively. Data were expressed as mean ± S.D. of three independent experiments. The statistical significance was analyzed and compared to the DMSO control by unpaired t-test or One-way ANOVA. Dunnett’s post-hoc analysis with P-value (P < 0.05 *; P < 0.01 **) was considered significant. The original images for western blot analysis were shown in “Supplementary information”.
