Figure 4
Ergosterol inhibited β-catenin movement and transcriptional activity. The overexpression of empty vector (EV) and wild-type β-catenin were transfected into (a) MCF-7 and (b) MDA-MB-231 cells. After 24 h of DMSO or ER 20 μM treatments, the β-catenin and c-Myc protein expression were detected by Western blots. (c) The β-catenin expression of transfected cells with β-catenin expression vector was visualized under confocal microscopy at 200X magnifications. The scale bar indicates 50 µM. The nuclear translocation of β-catenin was measured using Image J software. (d) The transcriptional activity of the β-catenin was performed by TOP/FOP flash luciferase reporter system with DMSO or ER 20 μM treatments after 24 h of incubation period. Data were expressed as mean ± S.D. of three independent experiments. The statistical significance was analyzed and compared to the DMSO control by unpaired t-test or One-way ANOVA. Dunnett’s post-hoc analysis with P-value (P < 0.05 *; P < 0.01 **) was considered significant. The original images for western blot analysis were shown in “Supplementary information”.
