Figure 6
The metabolic antibody array with Ergosterol treatment on MDA-MB-231 cells. (a) After treating MDA-MB-231 cells with DMSO or ER 20 μM for 24 h, whole cell lysates (200 μg) were collected and loaded into the metabolic antibody array, according to the manufacturer’s protocol. The antibody array was visualized using a chemiluminescence detector, representing the protein expression. (b) The internal positive controls were used to normalize the chemiluminescence signal between membranes, and the relative protein profiles were compared to the DMSO control. (c) Downregulated proteins were clustered based on their KEGG enrichment pathways using the STRING database. The clustered node was identified as steroid hormone biosynthesis (yellow), nitrogen metabolism (green), sphingolipid metabolism (navy), pyrimidine metabolism (red), and glycosaminoglycan biosynthesis (blue), respectively.
