Fig. 1
Confirmation of DCL knockdown in β-tanycytes lining the median eminence. DCL-KD mice and WT littermates were put on a dox-containing diet for 5 weeks to induce DCL knockdown in the transgenic mice. Confocal microscopy was performed in the hypothalamus of (A,C) WT and (B,D) DCL-KD mice to show and quantify (E) DCL expression (green) in the wall of the third ventricle (3v) (n = 6 per group). Arrows indicate DCL positive projections into median eminence (ME). Asterisks indicate areas of clearly reduced DCL expression. Scale bars measure 100 µm in (A) and (B), and 40 µm in (C) and (D). Data is presented as mean ± SEM. ***P < 0.001 according to unpaired t-test (E).
