Fig. 2
DCL-KD prevents body weight gain in mice, due to lowered fat mass accumulation. DCL-KD mice (black bars) and WT littermates (grey bars) were fed a dox-containing diet for five weeks to induce DCL knockdown in the transgenic mice. (A) Body weight was monitored over a period of 52 weeks during which the dox-containing diet was replaced by dox-free diet at week 32. At week 0 and week 6 (B) fat mass and (C) lean mass were measured by Echo-MRI (n = 6–8 per group). In a separate study, mice were put on dox diet for 5 weeks. During the first 4 days mice were single-housed in metabolic cages while (D) food intake, and (E) energy expenditure (expressed per g lean mass), (F) fat oxidation rate, and (G) carbohydrate oxidation rate were monitored. (H,I) Energy expenditure was plotted against lean mass and linear regression was performed. At week 5, (J) plasma thyroxine, (K) plasma triiodothyronine, and (L) hypothalamic D2 activity were determined either in the ad libitum fed state or after 48 h of fasting (n = 5–8 per group). Data is presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 according to unpaired t-test (A–C) or two-way ANOVA and following Ŝídák’s multiple comparisons test (E–G,J–L).
