Fig. 5

Effect of BJe on MAPKs protein expression in THP-1 cells. Cells were exposed to BJe at 0.5, 1 and 2.5 mg/mL for 48 h, respectively and were then processed for protein level assessment determined by Western blotting. On the left (A), immunoblots representative of three independent experiments are shown, while their densitometric analysis is displayed on the right (B). Levels of phosphorylated JNK, ERK and p38 were expressed as a ratio of phosphorylated forms over their total protein concentration. Results were extrapolated as fold change versus untreated cells, which are assigned as 1 arbitrarily and expressed as mean ± SEM, of three different experiments (N = 3). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 versus control (CTRL).