Table 2 JE2 mazF::tn becomes susceptible to beta-lactam antibiotic cefazolin when grown in mammalian serum. Cefazolin and vancomycin minimum inhibitory concentration (MIC) assays were performed on JE2 WT, JE2 mazF::tn, and JE2 mazF::tn with mazF expression restored using complement in Mueller Hinton Broth (MHB) and fetal bovine serum (FBS).

From: Methicillin resistant Staphylococcus aureus mazEF expression promotes infections by influencing cellular growth, antibiotic sensitivity, and formation of biofilms

S. aureus Strains

Treatment

MHB

FBS

JE2 WT

Cefazolin

> 96

0.5

JE2 WT

Vancomycin

1

1

JE2 mazF::tn

Cefazolin

1

1

JE2 mazF::tn

Vancomycin

0.5

1

JE2 mazF::tn + complement

Cefazolin

> 96

1

JE2 mazF::tn + complement

Vancomycin

0.5

1