Table 2 JE2 mazF::tn becomes susceptible to beta-lactam antibiotic cefazolin when grown in mammalian serum. Cefazolin and vancomycin minimum inhibitory concentration (MIC) assays were performed on JE2 WT, JE2 mazF::tn, and JE2 mazF::tn with mazF expression restored using complement in Mueller Hinton Broth (MHB) and fetal bovine serum (FBS).
S. aureus Strains | Treatment | MHB | FBS |
|---|---|---|---|
JE2 WT | Cefazolin | > 96 | 0.5 |
JE2 WT | Vancomycin | 1 | 1 |
JE2 mazF::tn | Cefazolin | 1 | 1 |
JE2 mazF::tn | Vancomycin | 0.5 | 1 |
JE2 mazF::tn + complement | Cefazolin | > 96 | 1 |
JE2 mazF::tn + complement | Vancomycin | 0.5 | 1 |