Fig. 1 | Scientific Reports

Fig. 1

From: Rapid identification of antibody impurities in size-based electrophoresis via CZE-MS generated spectral library

Fig. 1

A workflow for mAb impurity analysis combining electrophoretic separation and accurate assignment of masses (A) mAb impurities derived by forced degradation conditions emulating cell culture and formulation screening. (B) Analysis of impurity mass ladder derived from mAb using LabChip GXII electrophoresis and CZE-MS (C) CZE-MS spectral mass identifications of mAb impurities are mapped to the GXII peaks in the electropherogram via the calibration curve. (D) Reassignment of GXII peak identifications based on the accuracy of migration times using a lookup table of accurate CZE-MS masses. Note: graphics were created with BioRender.com.

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