Fig. 2
(A) Analysis of degradation products of NISTmAb A) LabChip GXII electropherogram of unknown mAb fragments peak 1–5 under unstressed (native condition), red trace and H2S stressed conditions green trace of NISTmAb. Inset is the intact H2L2 with with up to 3 trisulfide adducts. (B) Deconvoluted mass spectrum resulting from H2S-induced degradation products of NISTmAb. (C) Linear regression analysis via NISTmAb fragments, plotted as log10 of the NISTmAb fragment molecular weights (MW) versus the mobility or inverse of migration time. (D) Assignment of masses to GXII peaks based on calibration in C and assignment of peak 4 corresponding to HH. Note: NISTmAb intact molecule is denoted as H2L2, * is aglycosylated H2L2 and degradation products are denoted as L, H, HL, HH, HHL. Note: graphics were created with BioRender.com.
