Fig. 3
Analysis of pH-induced degradation products of Rituximab (A) Rituximab light chain sequence with site-specific NP cleavage. (B) CZE-MS mass spectrum of L-N/P Clip. (C) Overlayed LabChip GXII electropherogram corresponding to pH- and temperature-induced degradation products of Rituximab (Inset shows the intensity of peaks 1–8 under native Rituximab stored at 4 °C (blue), incubated at high-pH of 10 at 4 °C for 1 day (red) and incubated at high-pH of 10 at 4 °C for 2 days (green). (D) Assignment of NP cleavage products of Rituximab to peaks in the electropherogram with identities or masses obtained from the regression analysis. Note: Peak 6~90 kDa, * ~ 1 kDa standard and ** is a system peak from the fluorescent dye.
