Fig. 4
Analysis of Cathepsin D- and Cathepsin L-induced degradation products of NISTmAb (A) NISTmAb light chain sequence with site-specific AA cleavage and FI cleavage. Cathepsin-D and -L cleavages of light chain. (B) Overlayed LabChip GXII electropherogram corresponding to incubation of NISTmab with Cathepsin D at pH 5 (red), Cathepsin L at pH 3 (blue) and Cathepsin L at pH 7 (green) for 1 day (D1) and 2 days (D2) (Inset shows the intensity of peaks 1–10 for cathepsin degradation products at D1 and D2) (C) Assignment of Cathepsin cleavage products of NISTmAb to peaks in the electropherogram with masses obtained from the regression analysis. Note: In addition to peaks 1–10, additional peaks are observed in the electropherogram.
