Fig. 5
GXII peak assignment via CZE-MS identification of cathepsin degradation products of NISTmAb (A) Extracted ion chromatograms of AA, FI and FL clips. (B) Deconvoluted mass spectrum of FI Clip (C), Deconvoluted mass spectrum of AA Clip (D) Deconvoluted mass spectrum of and FL Clip (E). Mass spectrum of Cathepsin L-induced AA Clip shown in brown circles. Inset shows the isotope distribution of Z = + 8 ion). (F) CZE-MS/MS product ion spectrum of AA Clip resulting from collision induced dissociation of Z = + 8. Inset shows the fragment ion map of the AA Clip. (G). Assignment of a new peak associated with FL Clip along with electropherogram peak assignments from Fig. 4C.
