Fig. 4

YG8R mice exhibit a decrease in lipolysis capacity in adipose tissues rather than systemic abnormal glucose metabolism at a young age. (A–N) Y47 and YG8R male mice at 16 weeks old were fed normal chow since birth. Then they were euthanized after 24-h fasting (n = 6). The samples of blood and adipose tissue were collected. (A) Glucose tolerance tests (GTT) to show the blood glucose following a-bolus-of-glucose administration, performed in mice before euthanizing. (B) Area of the under curve (AUC) calculation by analysis of the GTT data in (A) with subtraction of basal glucose contents. (C) Postprandial blood glucose levels in mice. (D) Serum TAG content. (E) Fasting plasma glucose. (F) Fasting insulin levels. (G) Calculated HOME-IR values based on fasting plasma glucose and fasting insulin levels in mice. (H) ITT to show the levels of blood glucose following insulin administration. (I,J) Serum glycerol and FFAs. (K,L) Expression of genes related to lipolysis in iWAT and eWAT of Y47 and YG8R mice, respectively. (M,N) Total and phosphorylated Hsl levels in iWAT and eWAT, revealed by western blot analysis. Data were normalized to the Y47 mice. Values are shown as mean ± SD. t-test was used for significance. *P < 0.05, **P < 0.01, ***P < 0.001.