Fig. 3

Temporal changes in fasting blood glucose and glucagon after ten weeks of feeding with a high-fat, high-monosaccharide, low-fiber western diet. The WD resulted in a significant 16.5% increase in fasting blood glucose, approaching the upper physiological limit. This was accompanied by a trend towards lower serum glucagon levels which did not reach statistical significance. Box plots represent the 25^th, 50^th and 75^th percentile of the data ± 1.5 IQR (interquartile range). •••: P ≤ 0.001. Summary box plots produced using the ggplot2 package in R version 4.2.2.