Fig. 2 | Scientific Reports

Fig. 2

From: Derivation of human primary prostate epithelial cell lines by differentially targeting the CDKN2A locus along with expression of hTERT

Fig. 2

Cas9-CRISPR effectively targeted the CDKN2A locus. (a) Schematic representation of the CDKN2A locus and primer sets flanking the sgRNA target sites. (b) PCR amplification of the CDKN2A locus with primer sets flanking predicted Cas9 cut sites. Genomic DNA obtained from each clone and PrEC was amplified with the primer sets indicated above and resolved by agarose electrophoresis. A DNA ladder is shown in the first lane of the Exon 2 gel. All products match their predicted sizes. Gel Images are cropped for clarity. Images corresponding to full-length gels are shown in Suppl. Figure 1B. (c) hTERT is overexpressed in all the cell lines. hTERT mRNA expression was determined by RNA-Seq as described in the text and Fig. 6. Represented is the variance stabilization transformed normalized average (VST-averages) for hTERT in PrEC compared to all the immortalized cell lines.

Back to article page