Fig. 8 | Scientific Reports

Fig. 8

From: Derivation of human primary prostate epithelial cell lines by differentially targeting the CDKN2A locus along with expression of hTERT

Fig. 8

Comparative analyses of PrEC immortalized Clones 1, 2, 3 and p16-KO growing in 3D Cultures. (a) Representative images of each immortalized hPrEC clone and ATCC hPrEC P5 seeded in between two layers of Matrigel in iBidi μ-slide for 9 days (clones) or 12 days (PrEC). Organoid images taken using EVOS inverted microscope, at indicated fields. Note the formation of lumens in each clone. (b) Representative images of each PrEC immortalized clone and ATCC hPrEC P5 seeded between two layers of Matrigel and stained with laminin, vimetin (EMT marker), CK5 (Basal marker), Integrin B, E-cadherin (Epithelial Marker), CK18 (Luminal Marker). All organoids were also stained with phalloidin (F-actin) and DAPI. Images taken at 63 × objective (Zoom factor .75) using Leica TCS SP8 Confocal Microscope.

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