Table 4 Antibodies used for immunofluorescence.

From: Derivation of human primary prostate epithelial cell lines by differentially targeting the CDKN2A locus along with expression of hTERT

 

Company

Catalog#

Application

Primary antibody

 Integrin b1

Santa Cruz

sc-59827

IF

 Laminin b1

Santa Cruz

sc-33709

IF

 Vimentin

abcam

ab92547

IF

 E-Cadherin

CST

14472S

WB, IF

 Keratin 5

BioLegend

905503

WB, IF

 Keratin 18

BioLegend

628401

WB, IF

 Phalloidin-iFluor 555

Conjugate

abcam

ab176756

IF

Secondary antibody

 Goat anti-Rat IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488

Invitrogen

A11006

IF

 Goat anti-Rabbit IgG (H + L) Highly Cross- Adsorbed Secondary Antibody, Alexa Fluor 488

Invitrogen

A11034

IF

 Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647

Invitrogen

A21235

IF